Principles of Plant Biotechnology

Introduction to biotechnology, History of plant tissue culture and Plant genetic engineering, Plant cell and tissue culture; Different techniques used for sterilization in plant tissue culture, nutritional requirement of tissue culture, types of media, totipotency and morphogenesis, micropopagation, somaclonal variation, anther/pollen culture, embryo culture, endosperm culture, somatic embryogenesis, artificial seed/synthetic seed production, invitro pollination and fertilization, protoplast culture, somatic hybridization; genetic engineering: Method of cloning DNA in bacteria, Restriction enzymes, vectors for gene transfer, isolation of DNA fragments, polymerase chain reaction (PCR), molecular markers, DNA finger printing, methods of gene transfer, genetic engineering for insect resistance and male sterility.