General Enzymology

Structures and functions of vitamins and coenzymes; Classification and nomenclature of enzymes; Genetics of enzymes; Inhibition mechanisms in enzyme-catalyzed reactions.  Effects of temperature, PH, inhibitors etc on enzyme-catalyzed reactions.  Michaelis-Menten equation. Allosteric/regulatory enzymes; Active sites of enzymes; Estimation of kinetic parameters – enzyme activities, Vmax, Ki, etc. Zymogen activation; Digestive enzymes, etc.  Isolaton, purification and characterization of enzymes.  Recent advances in enzymology.